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Expression of Ep31. Putative epsilon subunit of coatomer protein complex isoform 2 (Ep31) of Angiostrongylus cantonens expressed in E. coli. Western blot analyses were performed using monoclonal anti-His tag (A), normal human (B) and Angiostrongylus-specific (C) serum. Lane 1 - Ep31 0.5 µg /lane; lane 2 - Ep31 0.3 µg/lane; lane 3 - Ep31 0.2 µg/lane; lane 4 - Ep31 0.1 µg /lane; lane 5 - crude extract of A. cantonensis 0.8 µg/lane. M - molecular weight in kDa.
