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. Author manuscript; available in PMC: 2013 Nov 8.
Published in final edited form as: Neuroscience. 2012 Aug 23;224:70–80. doi: 10.1016/j.neuroscience.2012.08.036

Fig. 1.

Fig. 1

Experimental design. The behavioral training consisted of 10 sessions over the course of two weeks. Sessions were conducted in behavioral chambers, and each consisted of eight pairings of a tone with food pellet delivery (conditioned group, Paired; n=20) or the same number of tones and food pellets presented at random (control group, Unpaired; n=20). Rats were maintained on a food-restricted feeding regimen throughout training. Following training rats were allowed ad libitum access to chow (satiation), and then tested while sated. During testing rats remained in their home cages and were given 10 presentations of the tone over 5 min. One group of rats (food consumption group; n=8/experimental condition) was then allowed to consume food pellets ad libitum for 75 min, while a second group (brain analysis group; ORX: n=12/experimental condition, MCH: n=4/experimental condition) was left undisturbed for 75 min until sacrifice.