. 2013 Apr-Jun;5(2):104–117.

Table 1.

Details of primers used for RT-RCR

Gane	Sequence (sense/antisense)	Annealing temperature (°C)×cycle no.	Fragment size (bp)	Accession no.
O.PPARa
	F:5’-AGAACAAGGAAGCGGAAGTC-3’	58×28	199	FJ200440
	R:5’-ATCCCGTCTTTGTTCATCAC-3’	58×28	199	FJ200440
O.Collagen1
	F:5’-CCCAGAACATCACCTACCAC-3’	55×38	317	FJ200442
	R:5’-GGAGGGAGTTTACAGGAAGC-3’	55×38	317	FJ200442
O.Aggecan
	F:5’-TTGGACTTTGGCAGAATACC-3’	55×40	196	FJ200438
	R:5’-CTTCCACCAATGTCGTATCC-3’	55×40	196	FJ200438
O.Oct4
	F:-5’-CAATTTGCCAAGCTCCTAAA-3’	50×40	290	AY490804
	R:5’-TTGCCTCTCACTTGGTTCTC-3’	50×40	290	AY490804
O.Sox2
	F:5’-TGATACGGTAGGAGCTTTGC-3’	50×41	362	X96997
	R:5’-GGTCTCTAAAGGGGCAAAAG-3’	50×41	362	X96997
O.GAPDH
	F:5’-TGGCAAAGTGGACATCGTTG-3’	50×38	467	NM-001190390-1
	R:5’-GCGTGGACAGTGGTCATAAGTC-3’	50×38	467	NM-001190390-1