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. Author manuscript; available in PMC: 2013 Jun 21.
Published in final edited form as: Nat Chem Biol. 2012 Oct;8(10):831–838. doi: 10.1038/nchembio.1059

Figure 2.

Figure 2

C18-Pyr-Cer mediates targeting of autophagolysosomes to mitochondria and the inhibition of mitochondrial function. (a) Targeting of autophagolysosomes to mitochondria in the absence or presence of C18-Pyr-Cer (C18PC) at 2 h in UM-SCC22A cells was examined by visualizing the co-localization of MTG and LTR using live cell imaging and confocal microscopy. Scale bars represent 10 microns. (b) Effects of C18-dihydro-Cer-14-piperidine (Dihydro-C18PC, left panel) on OCR were measured using the SeaHorse compared to C18-Pyr-Cer (C18PC) and vehichle-treated controls (right panel). Data shown are an average of at least two independent experiments performed in duplicates ± s.d. (*P <0.05).