Table 1.

The primers and primary antibody used in this study

Gene symbles	Primers for RT-PCR			Antibodies for IHC
		Primer sequences	Annealing temperature (°C)	Antibody sources	Clone	Dilution
Bmi1	Reverse	5’-ATT GTC TTT TCC GCC CGC TT-3’	58.2	ProMab Biotechnologies Inc	3E3	1:800
	Forward	5’-TGG CAT CAA TGA AGT ACC CTC-3’
CD44	Reverse	5’-TGC TAC TGA TTG TTT CAT TGC G-3’	56.2	ProMab Biotechnologies Inc	8E2F3	1:30000
	Forward	5’-GGA CCA GGC CCT ATT AAC CC-3’
CD133	Reverse	5’-AAA CAA TTC ACC AGC AAC GAG-3’	54.1	ProMab Biotechnologies Inc	3 F10	1:400
	Forward	5’-TAG TAC TTA GCC AGT TTT ACC G-3’
Sox2	Reverse	5’- GCT AGT CTC CAA GCG ACG AA-3’	56.2	ProMab Biotechnologies Inc	10 F10	1:800
	Forward	5’- TAC AGT CTA AAA CTT TTG CCC TT-3’
Nanog	Reverse	5’-AGG CAA CTC ACT TTA TCC CAA-3’	54.1	Cell signaling technology	D73G4	1:300
	Forward	5’-GAT TCT TTA CAG TCG GAT GCT T-3’
Oct-4	Reverse	5’-TGC AGA AAG AAC TCG AGC AA-3’	56.2	Santa Cruz Biotechnology	C-10	1:50
	Forward	5’-CTC ACT CGG TTC TCG ATA CTG G-3’
Msi2	Reverse	5’-CAG ACC TCA CCA GAT AGC CTT-3’	56.2	ProMab Biotechnologies Inc	2C11	1:1000
	Forward	5’-TAC TGT GTT CGC AGA TAA CCC-3’
β-actin (217 bp)	Reverse	5’GTG ACG TGG ACA TCC GCA AAG-3’	60.2
	Forward	5’-ATC CAC ATC TGC TGG AAG GTG GAC-3’
β-actin (417 bp)	Reverse	5’-ACA GAG CCT CGC CTT TGC CGA TC-3’	60.2
	Forward	5’-TGG GTC ATC TTC TCG CGG TTG G-3’