Figure 3.
Combined liquid phase IEF and SDS gel electrophoresis prefractionation (Variant 1). a: Vitreous protein sample VP2 was prefractionated by liquid phase IEF in a Zoom FractionatorTM (Invitrogen) yielding 5 fractions representing the indicated IP ranges. Each individual fraction was separated by SDS gel electrophoresis as described in the legend to Figure 2. The complete work flow is outlined as variant 1 in Figure 1. b: Overview of the total number of identified proteins in the different pH fractions obtained by variant 1. Details on the proteins are given in Additional file 1: Table S1.