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Meganuclease systems for SMG removal. Two I-SceI sites were designed to flank P1-SMG-T1 cassette. When I-SceI expresses, double-strand breaks (DSBs) are generated at both I-SceI sites and release the P1-SMG-T1 fragment. The broken ends are repaired through NHEJ repairing pathway. Abbreviations. RB: T-DNA right border, LB: T-DNA left border, GOI: gene of interest, SMG: selectable marker gene, P1 and P2: promoters, T1 and T2: terminators.
