Figure 9.

Strategy for SMG removal in transgenic plants with TALENs. A T-DNA containing GOI and 35S-SMG (flanked by two engineered identical TALEN-binding sites) are delivered into plant genome. Upon the expression of TALEN genes, TALEN nucleases bind to the TALEN-binding sites and induce DSBs, resulting in the release of the SMG fragment. The broken ends are repaired through endogenous repairing system. Abbreviations. RB: T-DNA right border, LB: T-DNA left border, GOI: gene of interest, SMG: selectable marker gene, P: promoter.