Table 3.
Quantification of RNA extracted from soil spiked with two concentrations of Pseudomonas sp. LBUM300 after multiple rounds of DNase I treatment
| Days post inoculation | Bacterial spiking amount |
RNA amount post DNase-I treatment (ng/μL)*¶ |
|||
|---|---|---|---|---|---|
| Round 0 | Round 1 | Round 2 | Round 3 | ||
| Day 0 |
Control |
33.7 ± 1.7 |
28.2 ± 2.4 |
25.2 ± 1.7 |
16.5 ± 2.2 |
| (-16.3%) |
(-25.2%) |
(-51.0%) |
|||
| 107 bacteria/mL |
53.9 ± 2.4 |
45.3 ± 3.1 |
40.1 ± 3.8 |
34.7 ± 4.2 |
|
| (-15.9%) |
(-25.6%) |
(-35.6%) |
|||
| 109 bacteria/mL |
102.4 ± 2.9 |
82.2 ± 4.4 |
61.7 ± 3.3 |
40.7 ± 4.9 |
|
| (-19.7%) |
(-39.7%) |
(-60.2%) |
|||
| Day 7 | Control |
34.4 ± 2.3 |
30.7 ± 2.8 |
26.8 ± 3.8 |
22.2 ± 4.5 |
| (-10.7%) |
(-22.1%) |
(-54.9%) |
|||
| 107 bacteria/mL |
56.6 ± 3.2 |
43.8 ± 4.4 |
32.7 ± 4.6 |
29.1 ± 3.5 |
|
| (-22.6%) |
(-42.2%) |
(-48.5%) |
|||
| 109 bacteria/mL | 59.5 ± 2.5 | 42.7 ± 3.2 |
34.9 ± 4.7 |
23.1 ± 5.4 |
|
| (-28.2%) | (-41.3%) | (-61.1%) | |||
* : Avg of 3 replicate samples ± standard error of mean.
¶: values in brackets indicate % reduction in RNA amounts (with respect to original starting amount), recovered after each round of DNase-I treatment.