Table 5.
A. fumigatus secondary metabolite biosynthetic gene clusters determined by SMURF, antiSMASH and by manual annotation or experimental characterization
|
A. fumigatus cluster summary | |||||||
|---|---|---|---|---|---|---|---|
| Backbone enzyme | SMURF | antiSMASH | Manual/experimental | Boundary 1 | Boundary 2 | Reference | |
| Fumigaclavine C (fga) cluster* |
Afu2g18040 |
Afu2g17930 - Afu2g18070 |
Afu2g17950 - Afu2g18070 |
Afu2g17960 - Afu2g18060 |
ED |
ED |
[48] |
| Fumitremorgin B (ftm) cluster* |
Afu8g00210 |
Afu8g00280 - Afu8g00100 |
Afu8g00100 - Afu8g00110 |
Afu8g00260 - Afu8g00170 |
ED |
ED |
[49] |
| Gliotoxin (gli) cluster* |
Afu6g09650 |
Afu6g09580 - Afu6g09740 |
Afu6g09520 - Afu6g09745 |
Afu6g09630 - Afu6g09740 |
ED, IGD |
ED |
[50] |
| Pseurotin A cluster* |
Afu8g00540 |
- |
Afu8g00342 - Afu8g00595 |
Afu8g005340 - Afu8g00570 |
ED |
ED |
[35] |
| Siderophore (sid) cluster* |
Afu3g03420 |
Afu3g03280 - Afu3g03580 |
Afu3g03270 - Afu3g03490 |
Afu3g03300 - Afu3g03460 |
ED |
ED |
[48] |
| Afu1g01010 cluster |
Afu1g01010 |
Afu1g00970 - Afu1g01010 |
Afu1g00970 - Afu1g01030 |
Afu1g00980 - Afu1g01010 |
FA |
IGD |
This study |
| Afu1g10380 (nrps1) cluster |
Afu1g10380 |
Afu1g10310 - Afu1g10380 |
Afu1g10310 - Afu1g10420 |
Afu1g10270 - Afu1g10380 |
ECS |
IGD |
This study |
| Afu1g17200 (sidC) cluster |
Afu1g17200 |
Afu1g17080 - Afu1g17240 |
Afu1g17090 - Afu1g17270 |
Afu1g17200 - Afu1g17240 |
ECS |
ECS |
This study |
| Afu1g17740 cluster |
Afu1g17740 |
Afu1g17710 - Afu1g17740 |
Afu1g17720 - Afu1g17750 |
Afu1g17710 - Afu1g17740 |
ECS |
ECS |
This study |
| Afu2g01290 cluster |
Afu2g01290 |
Afu2g01170 - Afu2g01400 |
Afu2g01210 - Afu2g01390 |
Afu2g01280 - Afu2g01330 |
ECS |
ECS |
This study |
| Afu2g05760 cluster |
Afu2g05760 |
Afu2g05730 - Afu2g05840 |
- |
Afu2g05740 - Afu2g05830 |
IGD |
IGD |
This study |
| Afu2g17600 cluster |
Afu2g17600 |
Afu2g17511 - Afu2g17600 |
Afu2g17490 - Afu2g17690 |
Afu2g17480 - Afu2g17600 |
IGD |
IGD |
This study |
| Afu3g01410 cluster |
Afu3g01410 |
Afu3g01400 - Afu3g01560 |
Afu3g01360 - Afu3g01560 |
Afu3g01400 - Afu3g01480 |
IGD |
IGD |
This study |
| Afu3g02530 cluster |
Afu3g02530 |
Afu3g02450 - Afu3g02540 |
Afu3g02450 - Afu3g02650 |
Afu3g02520 - Afu3g02650 |
FA |
IGD |
This study |
| Afu3g02570 cluster |
Afu3g02570 |
Afu3g02570 - Afu3g02650 |
Afu3g02450 - Afu3g02650 |
Afu3g02520 - Afu3g02650 |
FA |
IGD |
This study |
| Afu3g02670 cluster |
Afu3g02670 |
Afu3g02670 - Afu3g02760 |
Afu3g02670 - Afu3g02760 |
Afu3g02670 - Afu3g02720 |
ECS, IGD |
ECS, IGD |
This study |
| Afu3g12920 cluster |
Afu3g12920 |
Afu3g12960 - Afu3g12750 |
Afu3g13020 - Afu3g12820 |
Afu3g12960 - Afu3g12890 |
ECS |
ECS |
This study |
| Afu3g12930 cluster |
Afu3g12930 |
Afu3g13000 - Afu3g12750 |
Afu3g13020 - Afu3g12820 |
Afu3g12960 - Afu3g12890 |
ECS |
ECS |
This study |
| Afu3g13730 cluster |
Afu3g13730 |
Afu3g13730 - Afu3g13600 |
Afu3g13790 - Afu3g13600 |
Afu3g13750 - Afu3g13670 |
IGD |
FA |
This study |
| Afu3g14700 cluster |
Afu3g14700 |
Afu3g14880 - Afu3g14690 |
Afu3g14820 - Afu3g14620 |
Afu3g14730 - Afu3g14690 |
ECS |
FA |
This study |
| Afu3g15270 cluster |
Afu3g15270 |
Afu3g15290 - Afu3g15250 |
Afu3g15350 - Afu3g15190 |
Afu3g15290 - Afu3g15240 |
ECS, IGD |
ECS |
This study |
| Afu4g00210 cluster |
Afu4g00210 |
Afu4g00260 - Afu4g00210 |
Afu4g00290 - Afu4g00150 |
Afu4g00260 - Afu4g00200 |
IGD |
ECS |
This study |
| Afu4g14560 cluster |
Afu4g14560 |
Afu4g14730 - Afu4g14420 |
Afu4g14660 - Afu4g14440 |
Afu4g14610 - Afu4g14450 |
ECS |
ECS |
This study |
| Afu5g10120 cluster |
Afu5g10120 |
Afu5g10250 - Afu5g09970 |
Afu5g10240 - Afu5g10010 |
Afu5g10130 - Afu5g10040 |
ECS |
FA |
This study |
| Afu5g12730 cluster |
Afu5g12730 |
Afu5g12840 - Afu5g12720 |
Afu5g12830 - Afu5g12680 |
Afu5g12770 - Afu5g12730 |
FA |
ECS |
This study |
| Afu6g03480 cluster |
Afu6g03480 |
Afu6g03620 - Afu6g03430 |
Afu6g03550 - Afu6g03400 |
Afu6g03490 - Afu6g03430 |
ECS |
ECS, IGD |
This study |
| Afu6g08560 cluster |
Afu6g08560 |
Afu6g08540 - Afu6g08560 |
Afu6g08520 - Afu6g08640 |
Afu6g08550 - Afu6g08560 |
ECS |
ECS |
ECS, IGD |
| Afu6g12080 cluster |
Afu6g12080 |
Afu6g12040 - Afu6g12160 |
Afu6g11980 - Afu6g12145 |
Afu6g12040 - Afu6g12080 |
ECS |
ECS |
This study |
| Afu6g13930 cluster |
Afu6g13930 |
Afu6g13830 - Afu6g14050 |
Afu6g13820 - Afu6g14030 |
Afu6g13920 - Afu6g14000 |
ECS |
ECS |
This study |
| Afu7g00170 cluster |
Afu7g00170 |
Afu7g00200 - Afu7g00120 |
Afu7g00220 - Afu7g00100 |
Afu7g00190 - Afu7g00120 |
ECS |
ECS, IGD |
This study |
| Afu8g00540 cluster |
Afu8g00540 |
Afu8g00370 - Afu8g00370 |
Afu8g00490 - Afu8g00310 |
- |
n/a |
n/a |
This study |
| Afu8g00620 cluster |
Afu8g00620 |
Afu8g00640 - Afu8g00470 |
Afu8g00720 - Afu8g00390 |
- |
n/a |
n/a |
This study |
| Afu8g01640 cluster |
Afu8g01640 |
Afu8g01640 - Afu8g01640 |
Afu8g01600 - Afu8g01730 |
Afu8g01630 - Afu8g01640 |
ECS, IGD |
ECS, IGD |
This study |
| Afu8g02350 cluster |
Afu8g02350 |
Afu8g02460 - Afu8g02350 |
Afu8g02490 - Afu8g02280 |
Afu8g02430 - Afu8g02350 |
ECS, IGD |
ECS, IGD |
This study |
| No PKS or NRPS backbone 1 |
n/a |
- |
Afu4g11170 - Afu4g11300 |
- |
n/a |
n/a |
|
| No PKS or NRPS backbone 2 |
n/a |
- |
Afu4g11980 - Afu4g12070 |
- |
n/a |
n/a |
|
| No PKS or NRPS backbone 3 |
n/a |
- |
Afu5g00100 - Afu5g04130 |
- |
n/a |
n/a |
|
| No PKS or NRPS backbone 4 |
n/a |
- |
Afu7g00230 -Afu7g00350 |
Afu5g00100 - Afu5g00135 |
ECS, IGD |
ECS, IGD |
This study |
| No PKS or NRPS backbone 5 | n/a | - | Afu7g01180 - Afu7g01270 | Afu7g00260 - Afu7g00270 | ECS, FA | ECS | This study |
* Experimentally characterized; Abbreviations: ECS, End of cluster synteny; IGD, Increase in intergenic distance; FA, Change in functional annotation; ED, Experimentally determined (1).