Figure 3.

Regulation of core gene-expression modules by memory precursor cells. (a) Flow cytometry of CD8⁺ T cells from Id2-deficient mice (Id2-KO) and Id2-wild-type mice (Id2-WT) and Id3^hi and Id3^lo CD8⁺ T cells, on day 6 of infection with VSV-OVA. Numbers in quadrants indicate percent cells in each. (b) Heat map of the frequency of enrichment of clusters (Fig. 1c) in wild-type and Id2-deficient samples collected on day 6 of infection with Lm-OVA and Id3^hi and Id3^lo samples collected on day 5 of infection with VSV-OVA (both pre-peak time points) for the following comparisons: Id2-deficient versus wild-type cells (both KLRG1^loIL-7R^hi; top), KLRG1^loIL-7R^hi cells versus KLRG1^hiIL-7R^lo cells (both wild-type; middle), or Id3^hi versus Id3^lo cells (both CD44⁺KLRG1^loIL-7R^lo; bottom). Numbers in map indicate proportion of cells with enrichment for that comparison; a frequency of 1.0 (red) indicates memory potential, and a frequency of 0.0 (blue) indicates effector potential. *P < 0.05 (χ² test). (c) ‘Volcano plots’ of the comparison of Id2-deficient KLRG1^lo cells versus wild-type KLRG1^lo cells (top), wild-type KLRG1^lo cells versus wildtype KLRG1^hi cells (middle), and Id3^hi cells versus Id3^lo cells (bottom), showing cluster-specific genes for each comparison. Numbers in bottom right and left corners indicate the number of genes in that region. Data are representative of three independent experiments with three mice per genotype (a) or three experiments with three independent samples from pooled spleens (b,c; n ≥ 3 per sample).