Figure 5.

Regulation of genes associated with activation state is independent of infection. (a) Heat map of all genes upregulated or downregulated more than twofold in pooled effector cells relative to their expression in pooled memory cells during infection with Lm-OVA (LM) or VSV-OVA (VSV) at matching effector or memory time points (above plots). (b) Direct comparison of expression at each time point after infection with Lm-OVA or VSV-OVA; numbers in top left and bottom right corners indicate number of genes with difference in expression of over twofold (blue lines as in Fig. 4a). (c) Change in gene expression after infection with Lm-OVA versus VSV-OVA at pooled effector time points (horizontal axis) versus that at pooled memory time points (vertical axis); red, genes upregulated after infection with Lm-OVA; blue, genes upregulated after infection with VSV-OVA; labels indicate infection-specific genes of interest. (d) Flow cytometry of OT-I CD8⁺ cells at day 6 of Lm-OVA infection or day 5 of VSV-OVA infection; numbers at top indicate median fluorescent intensity (MFI); numbers above bracketed lines indicate percent CTLA-4⁺ cells (left) or KLRG1⁺ cells (right) in gated populations. (e) Comparison of gene expression by pooled effector cells versus pooled memory cells after infection with Lm-OVA (horizontal axis) versus that comparison after infection with VSV-OVA (presented as in Fig. 4b). *P < 0.001 and **P < 0.00001 (t-test). Data are from three independent experiments with three mice (a–c,e) or four mice (d) per group.