Figure 8. Surface Plasmon Resonance analysis of the interaction of SpsD_167–519 with Fbg, Fn, α-elastin and cytokeratin 10.

Human Fbg (A), Fn (B) and α-elastin (C) were immobilized onto the surface of dextran chips. GST-human K10_544–563, (D) was captured onto a sensor chip coated with goat anti-GST IgG. Representative sensorgrams display binding of SpsD_167–519 to and dissocation from Fbg, Fn, α-elastin and GST-human K10_544–563, in a single cycle kinetics assay. Binding was measured as response units (RU) against time. The affinities were calculated from curve fitting to a plot of the RU values against concentrations of SpsD. Arrows indicate the time at which SpsD_167–519 was injected. The data shown is representative of 3 individual experiments.