Figure 9. Binding of SpsD_{167 –519}, SpsD_{167 –519} F288A and SpsD_{167 –519} latch mutant to immobilized Fbg, Fn, α-elastin and K10.

Microtiter wells were coated with human Fbg (A), Fn (B), α-elastin (C) and K10 (D) in a bicarbonate buffer overnight. The wells were probed with increasing concentrations of SpsD proteins followed by incubation with mouse anti SpsD_37–519 IgG and HRP-conjugated rabbit anti-mouse IgG. Values are the means ± S.E of triplicate wells and are representative of one experiment. This experiment was performed three times with similar results.