Figure 5. TLR4 activation by (upLPS) suppresses airway eosinophilia induced by B. tropicalis allergens.

C57BL/6 (WT), TLR4^−/−, or TLR2^−/− mice were sensitized twice with Bt or Bt containing upLPS co-adsorbed to alum and challenged twice with Bt. Control group consisted of non-manipulated animals. The experiments were performed 24 h after the last Bt challenge. Total bronchoalveolar lavage (BAL) leukocyte counts (A), eosinophil counts (B) and neutrophil counts (C) in BAL fluid. Results are expressed as mean ±SEM for groups of five mice and are representative of two experiments. *Significant difference (p<0.05) when compared with Bt group.