Figure 2. 1,25(OH)₂D₃-elicited transactivation of target gene CAMP by VDR is influenced by ethnicity.

Box plots illustrate expression of VDR target genes, CAMP (A) and CYP24A1 (B). Data is differentiated by ethnicity: Africans (grey, n = 40) and Whites (white, n = 20). Gene expression was quantified in monocyte-macrophages from healthy individuals using RT-qPCR. In vitro 1,25(OH)₂D₃ supplementation significantly induced CAMP and CYP24A1 expression in both Africans and Whites relative to the vehicle control level (P<.050, P<0.010, P<0.001). Ethnicity had a significant main effect on CAMP (P<0.010), but not CYP24A1 mRNA level. Significantly higher mean CAMP mRNA level was observed in Whites compared to Africans after 1,25(OH)₂D₃ supplementation (* P<0.050). CAMP and CYP24A1 mRNA data was ln-transformed to meet the assumptions of parametric statistical analysis. Outliers are defined in legend for Fig. 1.