Figure 2. NAC treatment reverses the activation of FoxO3a.

A. Cells were treated with 25 mM glucose+NAC for 72 h and the Western blot probed with FoxO3a antibody. Representative Western blots data showing FoxO3a protein expression. B. Densitometric analysis of the Western blot obtained in three separate experiments. Data shows NAC-mediated protective effect against oxidative stress by glucose. The β-actin was shown as loading control.