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. 2013 Jun 21;8(6):e66717. doi: 10.1371/journal.pone.0066717

Figure 3. Loss of pAA by EHEC O104:H4 outbreak strain leads to the loss of the aggregative “stacked-brick” adherence to HCT-8 cells.

Figure 3

HCT-8 monolayers were incubated with overnight cultures of initial or follow-up isolates from patients A, C and F for 3 h in the presence of 0.5% D-mannose. The cells were washed, fixed and stained with 10% Giemsa. Bacterial adherence patterns were examined using light microscopy (AxioImagerA1; Zeiss, Jena, Germany). (A, C, E) adherence phenotypes of the initial pAA-positive isolates from patients A, C, and G, respectively. (B, D, F) adherence phenotypes of the pAA-negative follow-up isolates from the respective patients are shown. Data from one of three experiments are shown. Bar = 10 µm.