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. 2013 Jun 21;8(6):e67599. doi: 10.1371/journal.pone.0067599

Figure 6. Susceptibility to PK digestion.

Figure 6

PK susceptibility was analyzed by PTA-WB. (A) Influence of final PK concentrations: 0, 25, 50, 250, 500 and 1000 µg/ml. The intensity of the Western blot signals were determined, whereby 50 µg/ml, which is the standard PK concentration used in our protocol, was set as a reference point (100%). PK digestion was performed for 60 min at 55°C. (B) Degradation of the PrPSc in accordance to the length of PK digestion between 1 and 26 hours. 50 µg/ml PK was applied in all of the experiments. Signal intensity was measured and the signal obtained after 60 min PK digestion (standard protocol) was set as the reference point (100%).