Table 1. Forward and reverse primers used for the qRT-PCR of ADAM10, BACE1, PSEN2, PS2V and MAPT transcripts.

Gene	Forward Primer	Reverse Primer
RPS16	5′-AGACAGCTACAGCCGTGGCACAT-3′	5′-CAGAAGCAGAACAGGCTCCAGTAACTT-3′
ADAM10	5′-GTGATCGCCCAGATATCCAGT-3′	5′-GAACCCCATCATCAAAGTCTCG-3′
BACE1	5′-GAGATCGCCAGGCTCTGTG-3′	5′-CCACGATGCTCTTGTCATAGTTG-3′
PS2V (RT-PCR)	5′-ACGGTCAGCTTCATCCAG-3′ (in Psen2 exon 3)	5′-TCAGGAAGAGCGTGGGGTAA-3′ (in Psen2 exon 7)
PSEN2 (qPCR)	5′-CCGCTGCTACAAGTTCATCCA-3′	5′-CCACGTTGTAGGTCTTGAGCACT-3′
PS2V (qPCR)	5′-GCTTTCATCCACGGCTG-3′ (spans Psen2 exon 4/6 junction)	5′-CCGAGGTAGATGTAGGTGAAC-3′ (in Psen2 exon 6)
MAPT (RT-PCR for 3R and 4R)	(5′-ACTCCACCCAAATCACCCTCCTC-3′)	(5′-TTGATGCTGCCAGTGGAAGAGAC-3′)
MAPT (RT-PCR for 0N, 1N and 2N)	(5′- TTCTCCTCCACTGTCCTCTTCTG-3′)	(5′- GTGTCTCCAATGCCTGCTTCTTC-3′)
MAPT (qPCR for full length)	5′-TCCACCGAGAACCTGAAGCA-3′	5′-GATGTTGCCTAGCGAGCGG-3′
MAPT (qPCR for 3R)	5′-GGAAGGTGCAAATAGTCTACAAACC-3′	5′-CGCTCGCTAGGCAACATCTC-3′
MAPT (qPCR for 4R)	5′-TAGCAACGTCCAGTCCAAGTGT-3′	5′-CGCTCGCTAGGCAACATCTC-3′

Note that guinea pig exon designations are according to the cognate exons in human since annotation of the guinea pig genome sequence is currently rudimentary.