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. 2013 Jun 21;8(6):e66598. doi: 10.1371/journal.pone.0066598

Figure 5. LIG treatment changes the expression level of the Rho GTPases in T98G cells.

Figure 5

A) High concentration T98G cells were treated with 0.5% IPA or 5 µM LIG for 20 hours and then analyzed by Western blotting against RhoA, Rac1 and Cdc42. A housekeeping protein, GADPH, was also detected as a reference. The results were normalized using the control condition (no treatment) as reference. B) Similar tests were performed with low concentration T98G cells (single cell assay conditions) and 5 hours of incubation under 0.5% IPA or 5-µM LIG treatment. Error bars represent the standard error from two experiments.