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. 2013 Jun 21;8(6):e66234. doi: 10.1371/journal.pone.0066234

Figure 8. GRK2 Knock-down in Cultured Cardiomyocytes Reduces Cleaved Caspase 3 Levels and Increases Activated Akt and Bcl-2 Levels.

Figure 8

(A) Representative Western blot for GRK2, total Akt, phospho-Akt (as in Fig. 7), Bcl-2, BCl-xL, cleaved caspase-3 and GAPDH as a loading control cultured neonatal rat ventricular myocytes (NRVMs) following 3 day treatment with control or GRK2-specific siRNA (see Methods) under basal conditions or after a challenge with Chelerythrine (Chele, 10 µM). (B) Quantitative assessment of GRK2 levels in NRVMs 3 days after siRNA treatment (control or GRK2 specific siRNA), *p<0.05 GRK2-siRNA vs. Control-siRNA (ANOVA, n = 4 per group). (C–F) Quantitative assessment of protein levels of phosphor-Akt normalized to total Akt (C); Bcl-2 (D); Bcl-xl (E); and cleaved caspase-3 (F) from the corresponding NRVMs, *p<0.05 between groups as indicated in each graph, n = 4.