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. 2013 Jun 21;8(6):e69255. doi: 10.1371/journal.pone.0069255

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© 2013 Li et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

The rabbit polyclonal antiserum raised against the coat protein (CP) of Potato virus H–Ho and the detection antibodies (Agdia, Indiana USA) against Potato virus M (PVM), Potato virus S (PVS), and Potato latent virus (PoLV) were used in the analysis. The purified PVH (PurPVH), purified pET-CP^PVH expression product (CP^PVH-his), pGEX-CP^PVM expression product (CP^PVM), pGEX-CP^PVS expression product (CP^PVS), and the lyophilized positive control for PoLV (PoLV) (Agdia, Indiana USA) were used in the tests. Crude extracts from uninfected leaves of potato and Nicotiana glutinosa (N. g.) were used as the negative controls.