Table 1. Polymerase chain reaction primers used for the amplification and sequencing of the PVH genome.

Primer name	Sequence (5' to 3')	Nucleotide position	Polarity	Method
PoleR5	CYCAKYTCCATSWYYC		Antisense	Reverse transcription
PL001F	ACAAAAGAATACCAGGAGGAATTGC		Sense
PLN1674R	GACTTGTGGAGGCTTGTGAAAG		Antisense
Carla114F	ATGGCWYTNACWTAYAGRASKCC	092-114nt	Sense	Degenerate PCR
Carla900F	GGACGATGGGCAGGTGTAC	855-873nt	Sense
Car1340F	GTGGCGCTTGATAGGAAATC	1468-1487nt	Sense
Carla1763F	GCCCGTTACAGTCGCGCCAG	1756-1775nt	Sense
Carla3357R	GCTAATTTCGTAGACCAGAGAG	3336-3357nt	Antisense	Specific primer
Carla3226F	CTCGAGTACCTAGAGAGGAAGAG	3226-3248nt	Sense	Specific primer
Carla3600R	GTAGTTCGATGCCTCATGC	4839-4857nt	Antisense
Carla4211R	CTATTCTCGAATTTTGGGTTG	3954-3974nt	Antisense
Carla4610F	CAAGGTGGGTGCACAAAATC	4604-4623nt	Sense
Carla5040R	CTATATCCACTAACCAATCCCTGC	5006-5029nt	Antisense
Carla5468F	CCATGGCCAATATGCTTTTCAC	5468-5489nt	Sense	Specific primer
Carla5672R	CCATCAGCGCATAAGTTCCACC	5651-5672nt	Antisense	Specific primer
Carla5800F	CAGATTACACTTGCAACAGAAG	5029-5050nt	Sense
Carla6670F	GGCTGACGACAAGAAGGG	7170-7187nt	Sense
HC511-18TR	GG A T A T CTGCAG G A T C CAAGC(T)18	PolyA	Antisense	Reverse transcription
HC511R	GG A T A T CTGCAG G A T C CAAGC		Antisense	Anchored primer

^a The underlined sequences are the restriction sites specific for EcoRV (GAT↓ ATC) and BamHI (G↓ GATCC). Protection bases are included in the 5' termini.