Table 4. Detection of flow-sorted C. hominis oocysts using qPCR1.
| Primer/Probe set2 | ||||||
| Cryptosporidium spp. specific | C. hominis specific | |||||
| # Oocysts | JVA | CRU18S | JVAG1 | Ch003 | CRULib13Ch | |
| 1000 | 30.21±0.59 (12/12) | 33.65±1.47 (11/12) | 33.75±1.23 (12/12) | 32.33±1.54 (12/12) | 35.43±1.69 (12/12) | |
| 100 | 35.08±2.15 (11/12) | 36.19±1.36 (9/12) | 37.38±0.59 (9/12) | 36.06±1.28 (9/12) | 37.72±1.01 (8/12) | |
| 10 | 36.62±1.48 (8/12) | 37.32±1.34 (6/12) | 37.25±0.71 (2/12) | 37.70±0.45 (3/12) | 38.23 (1/12) | |
| 5 | 37.71±1.59 (5/12) | 38.87±0.43 (4/12) | 39.07±0.49 (2/12) | 36.77±0.26 (2/12) | 38.93 (1/12) | |
| 2 | 36.78±1.31 (4/12) | 38.10±1.10 (2/12) | * | 38.31 (1/12) | 38.81 (1/12) | |
| 1 | 38.71±0.72 (6/12) | 39.14±0.18 (2/12) | 39.06 (1/12) | * | 38.68 (1/12) | |
| 03 | * | * | * | * | * | |
1
Results presented are the average ± standard deviation of four independent experiments. QPCR was performed in triplicate reactions for each experiment.
Flow-sorted oocysts spiked in reagent water were used to determine detection limits of the different qPCR assays evaluated.
2
CT values ± standard deviation. Numbers in parentheses indicate the number of positive samples (CT values <40) over the total reactions performed.
3
“Mock” flow-sorted sample containing no oocysts.
*
, Not detected (CT >40).