Figure 4. Southern blot analysis of Alu-PCR amplicons.

Four sets of Alu-PCR were performed (See Table 1). (A and B) Alu3-L, Alu3-R, Alu5-L, and Alu5-R were the PCR products generated using primers Alu3/PCFo, Alu3/PCRo, Alu5/PCFo, and Alu5/PCRo respectively as the first stage PCR primers and Tag3/PCFi, Tag3/PCRi, Tag5/PCFi, and Tag5/PCRi respectively as the second stage PCR primers. The filter was hybridized with a digoxigenine-labeled HBV specific probe.