Figure 6.

Effect of perturbing the Cic gradient. (a) Simultaneous staining of Cic in wild-type and cic[1] embryos reveal very low level of Cic this mutant. (b) Quantified nuclear Cic in the posterior half of the wild-type embryo (red, N = 21) and embryos from cic[1] flies (black, N = 14). Error-bars are s.e.m. (c) Assuming that Cic is downregulated completely at the posterior pole of cic[1] embryos, we obtained normalized Cic gradient in wild-type embryos (red, N = 21), which show that Cic is degraded to approximately 20% of the maximum at the posterior pole. Similar approach was used to determine normalized gradient from cic heterozygous embryos (green, N = 33) and embryos with two extra copies of cic (blue, N = 25). Error bars are s.e.m. (d) Nuclear gradient of Cic in the posterior half of wild-type (red, N = 30) and embryos with two extra copies of cic (blue, N = 32). Filled yellow and green arrows denote the boundaries of tll and hkb, respectively, in wild-type embryos while empty arrows represent the two gene expression boundaries in the mutant embryos.