Panels A, B and C, MenA, W or X isolates, respectively, from cases of disease isolated during epidemics in sub-Saharan Africa. Panel D, MenB isolates from industrialized countries. The MenA, X and B strains had fHbp amino acid sequence variants in variant group 1 (See Table 1). The MenW stains had fHbp in variant group 1 (ID 9, ID 350) or variant group 2 (ID 23, ID 22). Bars represent the reciprocal GMT and ranges of three serum pools per vaccine group, and two pools for the adjuvant alone (four individual sera in each pool). Black bars, control mice immunized with aluminum hydroxide without a vaccine antigen; orange bars, control mice immunized with a NOMV-fHbp KO vaccine; white bars, mice immunized with the NOMV-fHbp vaccine.