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. 2013 Jun 21;8(6):e66376. doi: 10.1371/journal.pone.0066376

Figure 5. Effects of lignin and 6-BIO on differentiation of ES cells.

Figure 5

(a) Microscopic images of ES cells on day 4 of induction. ES cells were cultured with lignin (50 µg/mL) and various concentrations of 6-BIO (1, 2 and 4 µM). (b) Gene expression analysis by real-time PCR. Changes in the expressions of differentiation marker genes associated with the addition of lignin and 6-BIO were analyzed on day 4 of culture. The expression level of each marker gene was normalized to control. Scale bar: a = 200 µm Data are expressed as the mean ± SD of the three experiments. *P<0.05, **P<0.01, compared with the control.