Figure 6. Effects of lignin and 6-BIO on the differentiation of ES cells into ocular cells.

(a) Microscopic images of ES cells (Control). ES cells were seeded on ST2 cells and cultured in a melanocyte differentiation inducement medium. (b) Microscopic images of ES cells (lignin-added group). ES cells were cultured under lignin-added condition (50 µg/mL) in an ST2 cell-seeded melanocyte differentiation inducement medium. (c)Microscopic images of ES cells (lignin- and 6-BIO-added group). ES cells were cultured with lignin (50 µg/mL) and 6-BIO (4 µM) in a ST2 cell-seeded melanoctye differentiation inducement medium. Scale bar: a,b,c = 200 µm.