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. Author manuscript; available in PMC: 2013 Jun 21.
Published in final edited form as: Chem Asian J. 2011 Aug 24;6(10):2730–2737. doi: 10.1002/asia.201100411

Figure 1.

Figure 1

Macromer synthesis, crosslinking and cell encapsulation strategies. a) Ring-opening polymerization (ROP) of AzDXO initiated by PEG with the catalyst of 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU). [AzDXO] = 0.1125 M, [DBU] = 0.1 M, rt, Ar, 4 h; b) Synthesis of PEG-(DBCO)x by reacting DBCO-acid with PEG in CH2Cl2 under the catalysis of DIPC and DPTS, rt, 10 h; c) Depiction of the cell encapsulation by crosslinking PEG-P(AzDXO)2m and PEG-(DBCO)x via SPAAC “click” reaction; d) A representative demonstration of the rapid gellation of the cell-hydrogel constructs within 1 min of mixing the BMSC cell suspension (106 cells/mL) in a PEG20k-P(AzDXO)4 solution (10 w/v% in expansion media) and a 4-arm-PEG10k-DBCO solution (10 w/v% in expansion). The BMSC expansion media consisted of α-MEM without ascorbic acid and 20% FBS.