FIG. 1.

EphA4-Fc prevents ephrin-induced phosphorylation of EphA4. (A) Chinese hamster ovary (CHO) cells expressing EphA4 were blocked with EphA4-Fc prior to the addition of ephrin A4-Fc. Lysates were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and bands specific for total EphA4 and phosphorylated EphA4 detected. (B) Quantification of band densities shows that only in the absence of EphA4-Fc could ephrin A4-Fc significantly increase the phosphorylation of EphA4 above basal levels (one-way ANOVA with Bonferroni's post-test). Blocking with EphA4-Fc prevented ephrin-induced phosphorylation of EphA4. ***p<0.001.