FIG. 4.

MRI analysis shows that the area of the dorsal funiculus is greater following EphA4-Fc treatment after spinal cord injury (SCI). Examples of high-resolution MRI mid-sagittal views of injured spinal cords from rats treated with (A) vehicle alone (n=7), (B) 1 mg/kg (n=7), (C) 5 mg/kg (n=6), and (D) 20 mg/kg EphA4-Fc (n=8). (E) Example of a coronal MRI slice (from [B], white line indicates location), with regions of interest (ROIs) shown in (F). DF: dorsal funiculus; GM: gray matter; VL: ventrolateral white matter; C: cyst; and D: damaged tissue. (G–I) The area of the dorsal funiculus in rats treated with (G) 1 mg/kg, (H) 5 mg/kg, and (I) 20 mg/kg EphA4-Fc was significantly greater caudal to the injury epicenter when compared with controls. The area of the dorsal funiculus for rats treated with the vehicle is shaded to the extent of its standard error of the mean. (J) A schematic of the increased area of the dorsal funiculus caudal to the injury epicenter (striped area) in EphA4-Fc-treated rats compared with rats treated with the vehicle alone. Scale bars for panels (A–D) as indicated in (A), and for (E) and (F) as indicated in (F). *p<0.05, **p<0.01, two-way ANOVA with Bonferroni's post-test.