FIG. 6.

Quantitative and statistical analysis of phenotypic reversal: nestin. The percentage of nestin-positive cells at day 14 were calculated relative to cultures maintained in the original factor for 14 days. Panels A and B show the percentage of cells staining positive for nestin relative to the total number of cells. Panel A shows the quantitative analysis for glial restricted progenitors (GRP) differentiated with bone morphogenetic protein 4 (BMP-4) and indicates increased levels of the nestin marker on challenge with basic fibroblast growth factor. Panel B shows the quantitative analysis for GRP differentiated with ciliary neurotrophic factor and indicates reduced levels of the immature nestin marker on challenge with BMP-4. Error bars represent 1 standard error. Dashed lines have been added to provide reference to cultures of GRP differentiated for 14 days with an original treatment. Stars summarize the statistical analysis showing the significant changes among the groups compared to cultures grown for 14 days in the initial factor. Statistical significance was set at p<0.01 for all comparisons.