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Detection of the intersubunit disulfide bond in native mVSOP/Hv1. Macrophages (MΦ) were collected from the abdominal cavities of adult mice and dissolved in standard Laemmli buffer in the absence of reducing agents such as DTT and β-mercaptoethanol. Proteins were detected by Western blotting with anti-mVSOP/Hv1 antibody. The same protein samples incubated with DTT or copper phenanthroline (CuP) prior to electrophoresis were also analyzed.
