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PlGF isoforms expressed in HEK293 cells are biologically active. A and B, SDS-PAGE and Coomassie stain (A) and CD spectroscopy (B) of PlGF isoforms expressed in HEK293 cells. C, stimulation of HUVECs with PlGF isoforms (2.5 nm) and analysis of VEGFR-1 phosphorylation (Tyr-1213); the degree of phosphorylation was determined by densitometric analysis of phospho-signal intensity and normalized to the corresponding β-actin signal.
