FIGURE 6.

The HBD promotes PlGF-2-induced wound angiogenesis and recruitment of perivascular cells. A, representative immunohistochemical staining for endothelial cells (CD31) and perivascular cells (desmin) of PlGF-treated wound tissues in diabetic mice at day 14 postinjury. B, quantification of the area within the granulation tissue that stained positive for CD31 or desmin at days 10 and 14 postinjury. Coverage of vessels with pericytes was quantified by calculating the ratio of desmin/CD31 double stained area. The dotted line indicates epidermal-dermal junction. e, epidermis. Scale bars, 100 μm. PlGF proteins were expressed in HEK293 cells.