FIGURE 2.

mRNA of Has1–3 (A–C), Hyal1 (D), Hyal2 (E), and Cd44 (F) in epidermal keratinocytes show distinct temporal patterns after a single dose of UVB. The relative expression levels were determined by qRT-PCR at the specified time points after UVB irradiation (10 mJ/cm²). All mRNA levels were normalized to Rplp0. Each time point represents means and S.E. (error bars) from 3–4 independent experiments, each done in duplicate. The differences between control and UVB-treated specimens were significant for all genes studied (p < 0.001 for Has2, Has3, CD44, Hyal1, and Hyal2 and p < 0.05 for Has1; two-way analysis of variance). The results of Bonferroni's tests for individual time points are shown (*, p < 0.05; **, p < 0.01; ***, p < 0.001).