TABLE 1.

Primers used in qRT-PCR to analyze the expression of genes corresponding to TcCad1, TcSSS, TcAPN-I, TcAPN-II, and TcAPN-III proteins and to generate dsRNA in RNAi experiments

Accession number and name	Sequence
XP_971388
TcCad1 RNAi Fw	5′-GAATTGTAATACGACTCACTATAGGGAACTGACCAAATCACCTTCG-3′
TcCad1 RNAi Rv	5′-GAATTGTAATACGACTCACTATAGGTGTCTCCAACATCTTTATCGGT-3′
TcCad1 qRT-PCR Fw	5′-AACAACCCGAGTGGCGAAT-3′
TcCad1 qRT-PCR Rv	5′-TCTGCCATTGATGAGTTCTTGGT-3′
EFA03129
TcSSS RNAi Fw	5′-GAATTGTAATACGACTCACTATAGGCTTCCAAACTTACAGTAAAACTAG-3′
TcSSS RNAi Rv	5′-GAATTGTAATACGACTCACTATAGGAACACTGATAAGATATTGGTCC-3′
TcSSS qRT-PCR Fw	5′-AAACCGCGATTCTGGTAAACC-3′
TcSSS qRT-PCR Rv	5′-TGACCGAATTGTGGTATGGTGAT-3′
NP_001164285
TcAPN-I RNAi Fw	5′-GAATTGTAATACGACTCACTATAGGAGTCCACGATGTTTCTAGAGC-3′
TcAPN-I RNAi Rv	5′-GAATTGTAATACGACTCACTATAGGCAGTCAGTATGTTCAACGTCAG-3′
TcAPN-I qRT-PCR Fw	5′-CAAGTGGCGGTCCCAGAT-3′
TcAPN-I qRT-PCR Rv	5′-TCAACAATCCCCAATTTTCCA-3′
EEZ99297
TcAPN-II RNAi Fw	5′-GAATTGTAATACGACTCACTATAGGCGATAACAAGTTTGGTAACACG-3′
TcAPN-II RNAi Rv	5′-GAATTGTAATACGACTCACTATAGGAGATATTTGTTAAGTACGGCTTC-3′
TcAPN-II qRT-PCR Fw	5′-CGCCCTATTCGCCACTGA-3′
TcAPN-II qRT-PCR Rv	5′-CGACTATTTGTGCCCGGTTT-3′
EEZ99296
TcAPN-III RNAi Fw	5′-GAATTGTAATACGACTCACTATAGGCTGAAGTACTACTAGCAGCGG-3′
TcAPN-III RNAi Rv	5′-GAATTGTAATACGACTCACTATAGGCATCGCTTGCTAGAACAGG-3′
TcAPN-III qRT-PCR Fw	5′-CACCTGGTCTGTTCACGAAATG-3′
TcAPN-III qRT-PCR Rv	5′-CCCAACTTTTTCGGCCAAT-3′
EFA04159
TcRPS18 qRT-PCR Fw	5′-TGATGGCAAACGCAAAGTCA-3′
TcRPS18 qRT-PCR Rv	5′-TCGGCCGACACCTTTGA-3′