FIGURE 7.
Copper uptake of hCTR1 mutants. A, hCTR1 WT, MM, H139R, and AAA and combination mutants described under “Results” were expressed in HEK293 Flp-InTM T-RExTM cells and induced with tetracycline. 64Cu uptake assays were done in 10 μm total copper. Uptake values were normalized to cells overexpressing WT, which was set to 100%. Normalized values (“Experimental Procedures”) from three independent experiments are shown ± S.D. B, hCTR1 WT, H139R, H139R AAA, and AAA mutants were assayed for 64Cu uptake exactly as in panel A.