FIGURE 2.

The effect of integrin αIIbβ3 inhibition on Pyk2 phosphorylation induced by 2-MeSADP and AYPGKF. Platelets were stimulated with 100 nm 2-MeSADP (A) or 500 μm AYPGKF (B) at 37 °C for 2 min in the presence and absence of 10 μm SC57101. Equal amounts of proteins were separated by SDS-PAGE, Western blotted, and probed for anti-phospho-Pyk2 (Tyr-402) or anti-β-actin (lane loading control) antibodies. The blot shown is representative of three independent experiments. C and D, densitometric measurement of phospho-Pyk2, expressed as fold-increase over control. Data are mean ± S.E. (n = 3). *, p < 0.05.