FIGURE 5.

AYPGKF-induced platelet aggregation, secretion, and TxA₂ generation in Pyk2-deficient platelets. Non-aspirin-treated washed platelets from Pyk2^−/− mice and Pyk2^+/+ littermates were stimulated with different concentrations of AYPGKF at 37 °C for 3.5 min, and platelet aggregation (A), ATP secretion (B), and TxA₂ generation (C) were measured as described under “Experimental Procedures.” The arrow indicates when agonist is added. In some experiments, platelets were preincubated with 1 μm AG17 prior to platelet stimulation as noted. All data shown are representative of three independent experiments. Data are mean ± S.E. (n = 3). *, p < 0.05; **, p < 0.005 compared with wild-type.