FIGURE 7.
Effect of Pyk2 to TxA2 generation downstream of integrins and G12/13 pathways. A, non-aspirin-treated washed human platelets were pre-treated with AG17 (1 μm) at 37 °C for 5 min following co-stimulation with 100 nm 2-MeSADP and 10 μm epinephrine in the presence of 100 μm MRS2179 for 3.5 min under stirring conditions. Platelet aggregation and ATP secretion were measured by aggregometry. The arrow indicates when agonist is added. Tracings are representative of three independent experiments. B, the effect of Pyk2 inhibition on combined Gi and Gz stimulation on TxA2 generation. 10 μm SC57101 was added 1 min prior to the addition of agonists where noted. C, the effect of Pyk2 inhibition on G12/13-dependent TxA2 generation. Non-aspirin-treated washed human platelets were stimulated with 100 nm 2-MeSADP, 60 μm YFLLRNP, or 100 nm 2-MeSADP + 60 μm YFLLRNP as indicated for 3.5 min, and the effect of AG17 on TxA2 generation was measured. Data are mean ± S.E. (n = 3). *, p < 0.005.