FIGURE 1.
Kras G12D induces a highly penetrant T-ALL in the BALB/c genetic background. Lethally irradiated mice were transplanted with 2.5 × 105 total bone marrow cells from control or Kras G12D mice in a pure B6 or BALB/c background along with same number of competitor cells. A, Kaplan-Meier survival curves of reconstituted mice. Cumulative survival is plotted against days after transplantation. The p value was determined by the log-rank test. B, disease distribution patterns in recipient mice transplanted with Kras G12D cells. C, flow cytometric analysis of total thymocytes from representative mice with control cells and T-ALL mice with Kras G12D cells. D, flow cytometric analysis of peripheral blood from representative mice with control cells and myeloproliferative neoplasm (MPN) mice with Kras G12D cells using antibodies against Mac-1 (CD11b) and Gr-1 (Ly6C/Ly6G). The percentages of cells enriched for monocytes (upper left quadrant) and granulocytes (upper right quadrant) are indicated on the plots.