FIGURE 4.

Kras G12D thymocytes overexpress surface NOTCH1 but not Rag1/2 at the pre-leukemia stage. Recipient mice transplanted with control or Kras G12D cells were killed at 8 weeks (2 month) or at a moribund stage (∼12 weeks). A, semiquantitative RT-PCR was performed to detect Rag1 and Rag2 levels in control and Kras G12D thymocytes. B, representative histograms of surface expression of NOTCH1 in control and Kras G12D CD4⁺ CD8⁺ thymocytes at different T-ALL stages. Quantified data are mean ± S.D. over control cells. #, p < 0.05 (compared with controls); *, p < 0.05 (compared between different stages of T-ALL).