FIGURE 4.

The half-lives of endogenous LAMTOR3 and Myc₆LAMTOR3 are reduced in the absence of LAMTOR2. Cell lysates were separated by SDS-PAGE, analyzed by Western blotting, and probed with the indicated antibodies. A and B, quantifications of the according Western blot are shown in A and B representing the averages of three independent experiments (means ± S.E., n = 3). C, 80% confluent LAMTOR2^−/− and LAMTOR2^f/−MEF were treated with 50 μg/ml cycloheximide for the corresponding time points. The cell lysates were separated by SDS-PAGE, analyzed by Western blotting, and probed with the indicated antibodies. D, Myc₆LAMTOR3LAMTOR2^−/− and Myc₆LAMTOR3LAMTOR2^f/− stable clones were treated with 50 μg/ml cycloheximide for the corresponding time points.