FIGURE 8.

Redundancy in lysine contribution toward LAMTOR3 proteasomal degradation. Stability of the tested mutant proteins expressed in cycloheximide treated HeLa cells (80% confluent) is shown in A and B. 48 h after transfection, the cells were treated with 50 μg/ml cycloheximide for the corresponding times. The lysates were separated by SDS-PAGE, analyzed by Western blotting, and probed with the indicated antibodies. The graphs represent average of three independent experiments (means ± S.E., n = 3). C, HA-LAMTOR3K0. D, HA-LAMTOR3WT. E, HA-LAMTOR3CN. F, HA-LAMTOR3Nterm. G, HA-LAMTOR3Middle. H, HA-LAMTOR3Cterm.