FIGURE 7.

Inhibition of autophagy reduced the anti-tumor effect of nilotinib in vitro and in vivo. A, co-treatment with autophagy inhibitors, 3-methyladenine (3-MA, 1 mm) or hydroxychloroquine (HCQ, 10 μm), reduced the effect of nilotinib on cell death. Cells were treated with nilotinib at10 μm and/or 3-MA/HCQ for 24 h. Cell viability was analyzed by MTT assay. B, combination of nilotinib and HCQ reduced the effect of nilotinib on Huh-7 tumors. Points, mean (n = 6); bars, S.E. *, p < 0.05; **, p < 0.01. (nilotinib group versus nilotinib plus HCQ group). C, Western blot analysis of P-AMPK, AMPK, and LC-3 in Huh-7 tumors.