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Significance of Nrf2 phosphorylation by PKC. A, total cell lysates were immunoprecipitated using anti-IgG, anti-Siah2, or anti-Keap1 antibodies and then immunoblotted using anti-Nrf2 antibody. B, lysates were immunoprecipitated using anti-IgG, anti-Nrf2, or anti-Siah2 antibodies and then immunoblotted with anti-phosphoserine antibody. Four dots with cross, effect of transfection with the Siah2 pBAsi plasmid is indicated by HIF-1α blots. Ab, antibody.
