FIGURE 3.

Effect of hypoxia and/or MG132 on accumulation of Nrf2(93–604). Hep3B cells were transfected with the empty pcDNA3.1 vector (MOCK), pcDNA3.1 containing Myc-Nrf2, or Myc-Nrf2(93–604). Forty eight hours after transfection, the transfected and WT Hep3B cells were grown under normoxia or hypoxia for 6 h in the presence or absence of MG132 (5 μm). Total cell lysates were immunoblotted with anti-c-Myc, anti-HIF-1α, or anti-β-actin antibodies. Ab, antibody.